megatomi.com for Dummies
megatomi.com for Dummies
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Our new Crystal clear+ tissue clearing technique is the only approach that delipidates samples without transform in morphology and with negligible impact on structural integrity.
Megatome is really a vibrating microtome created to part a wide selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With higher blade vibrating frequency and minimized blade deflection, Megatome enables large-throughput tissue sectioning with uniform floor profile, as well as nominal tissue hurt and knowledge decline.
Antibodies may possibly consider months to diffuse by way of just a few millimeters of tissue, that has a steep labeling gradient from floor to Main.
Megatome is created for precision: the blade vibrates at a better frequency and bigger amplitude assortment than other microtomes, and incorporates a special deflection Handle mechanism.
SE uses a rotational electrical area to disperse highly electromobile molecules (such as antibodies or surfactant micelles) all over a porous sample without the need of detrimental electrically billed constructions within the tissue. This allows two-four day clearing of intact organs,
Our preformulated EasyIndex megatomi.com Resolution raises and homogenize the refractive index of delipidated tissue samples, rendering them completely clear. This enables light penetration into the sample and makes sure the acquisition of higher-resolution, in-emphasis graphic knowledge.
Megatome is usually a novel microtome that allows for high-precision sectioning of an array of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with small tissue problems and knowledge decline.
eFLASH is a rapid tissue labeling method that allows for uniform entire-organ staining in 20 rounds of labeling.
Totally delipidate total mouse brains or comparably sized samples in just one working day with SmartBatch+, or in one 7 days with our passive clearing package.
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Leverage the Distinct+ tissue clearing method, in addition to eFLASH and patented stochastic electrotransport systems, to fast clear and label entire organs. Essential highlights and capabilities involve:
Our novel SHIELD tissue preservation approach varieties intramolecular bonds applying polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.